The Effect of Different Single Doses from X-Rays on Pediatrics Rhabdomyosarcoma Tumor Cells: In Vitro Survival Curve

Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma of childhood. RMS tumor exhibits the skeletal muscle differentiation [1,2]. The two major histiotypes, embryonal and alveolar, are characterized by specific genetic abnormalities that provide clues to the mechanisms of tumor induction [1]. Contemporary risk-based therapy cures more than two thirds of children with RMS while minimizing acute and late effects [1]. To examine the capability of a group of single cells to grow and form colonies after being irradiated to different single radiation doses, reproductive integrity measure is a convenient proof that the cells retained their functions. The loss of this ability as a function of radiation dose is described by the dose-survival curve. A cell survival curve describes the relationship between the radiation absorbed dose and the proportion of cells that survive (or surviving fraction) [3,4]. For a tumor to be eradicated in a single fraction in radiotherapy, it is only necessary that cells be killed in the sense that they are rendered unable to divide and cause further growth and spread of the malignancy [3].